Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations. ... (where s is the standard deviation of the PSF) and the corresponding radiality transform (right) is depicted for the diffraction-limited intensity profile shown on the left (frames 1-60). Next (frames 61-104), the ellipticity of the. An ideal PSF should represent how a point emitter smaller than the diffraction limit of your optical setup is imaged by your microscope. PSFs can be be calculated theoretically, but this requires exact modeling of your microscope, so it is more common to use images of sub-diffraction fluorescent beads to estimate the PSF.. "/>. A community for the discussion of image analysis, primarily using ImageJ (and FIJI), a free, open source, scientific image processing and analysis program using Java, and is used worldwide, by a broad range of scientists.

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